The development of a high oil haloid inducer is partially funded through the SweetCAP project (NIFA SCRI Award 2018-51181-28419) in collaboration with the University of Florida.
Starting materials
BHI306
Specialty Inducer (ISURF#04099)
Selectable marker : R1-nj, Pl1
Average induction rate: 10-12%
OC ca. 4.5%
IHO
inbred line derived from the Illinois High Oil population (provided by S. Moose)
Oil content in the range of 15-18%
Performance of BC1 derived experimental lines (S2020):
Below are the results of test inductions with BC1-derived experimental inducer lines in summer 2020 onto a conventional hybrid (Viking 60-01N, Albert Lea Seed, MN). The induction rate (IR[%])of the experimental lines is in the range of 10-12%, their oil content lies around 10%. The resulting differences in haploid (HAP OC[%]) and diploid (HYB OC[%]) oil content vary in the range of 1%. All oil content measurements (OC[%]) were done by bulk NIRS.
| HO-IND | IR[%] | OC[%] | HAP OC[%] | HYB OC[%] | Δ OC |
|---|---|---|---|---|---|
| Exp01 | 10.8 | 11.3 | 3.8 | 5.0 | 1.2 |
| Exp02 | 10.8 | 11.4 | 4.0 | 5.1 | 1.1 |
| Exp03 | 12.8 | 9.6 | 4.1 | 4.8 | 0.7 |
| Exp04 | 10.8 | 9.8 | 3.7 | 4.9 | 1.2 |
Ongoing breeding efforts
To achieve a larger difference in oil content between haploid and diploid seed, BC2 derived lines are under development. We also started to recombine any germplasm fixed for the two major genes in haploid induction and the selectable marker R1-nj to generate a synthetic population, which will be selected over time for higher oil content. This population serves as a starting point for the development of inducer DH lines.
